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  Indian J Med Microbiol
 

Figure 1: Agarose gel electrograph of cytochrome c oxidase subunit I amplicon from human RNA; (-), RT negative control; EB, extraction blank; RTB, reverse transcription blank; AmpB, amplification blank (a), both DNA and RNA can be amplified in the polymerase chain reaction system, but amplification RNA extract is derived from RNA, since Lane 4 presents no polymerase chain reaction product band after Ambion-RNase cocktail treatment (0.05 U/μl-RNase A and 2 U/μl-RNase T1) at 37°C for 30 min. (b) Polymerase chain reaction products of primate blood RNA samples, with human RNA as positive control. PM Rhesus monkey; PM2-Spider monkey; PM3-black howler monkey; PM4-brown lemur, PM5-African green monkey; PM6-baboon; PM7-Cynomologous monkey; PM8-Spot nosed guenon; PM9-pig-tailed macaque; PM10-Chipanzee; (c) Polymerase chain reaction products of nonprimate blood RNA samples, with human RNA as positive control. Mouse-AM1; Duck-Am2; turtle-AM3; Opossum-AM4; Gopher Tortoise-AM5; Rabbit-AM6; Guinea pig-AM7; Alligator-AM8; Rooster-AM9; Frog-AM10; Calf-AM11; Cow-AM12; Dog-AM13; Cat-AM14; Horse-AM15; Deer-AM16; Pig-AM17; Goat-AM18; Sheep-AM19; Patagonian Cavy-AM20

Figure 1: Agarose gel electrograph of cytochrome c oxidase subunit I amplicon from human RNA; (-), RT negative control; EB, extraction blank; RTB, reverse transcription blank; AmpB, amplification blank (a), both DNA and RNA can be amplified in the polymerase chain reaction system, but amplification RNA extract is derived from RNA, since Lane 4 presents no polymerase chain reaction product band after Ambion-RNase cocktail treatment (0.05 U/μl-RNase A and 2 U/μl-RNase T1) at 37°C for 30 min. (b) Polymerase chain reaction products of primate blood RNA samples, with human RNA as positive control. PM Rhesus monkey; PM2-Spider monkey; PM3-black howler monkey; PM4-brown lemur, PM5-African green monkey; PM6-baboon; PM7-Cynomologous monkey; PM8-Spot nosed guenon; PM9-pig-tailed macaque; PM10-Chipanzee; (c) Polymerase chain reaction products of nonprimate blood RNA samples, with human RNA as positive control. Mouse-AM1; Duck-Am2; turtle-AM3; Opossum-AM4; Gopher Tortoise-AM5; Rabbit-AM6; Guinea pig-AM7; Alligator-AM8; Rooster-AM9; Frog-AM10; Calf-AM11; Cow-AM12; Dog-AM13; Cat-AM14; Horse-AM15; Deer-AM16; Pig-AM17; Goat-AM18; Sheep-AM19; Patagonian Cavy-AM20