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ORIGINAL ARTICLE
Year : 2019  |  Volume : 5  |  Issue : 3  |  Page : 123-129

Degradation of β-Actin mRNA and 18S rRNA in mouse spleen cells after death


1 Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security; Collaborative Innovation Center of Judicial Civilization, China; Key Laboratory of Evidence Science (China University of Political Science and Law), Ministry of Education, Beijing, China
2 Public Security Bureau of Huaian City, Jiangsu, China
3 Collaborative Innovation Center of Judicial Civilization, China; Key Laboratory of Evidence Science (China University of Political Science and Law), Ministry of Education, Beijing, China

Correspondence Address:
Dong Zhao
Institute of Evidence Law and Forensic Science, China University of Political Science and Law, Ministry of Education, No. 25, Xitucheng Road, Haidian District, Beijing 100088
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jfsm.jfsm_38_19

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We observed degradation of β-actin mRNA and 18S rRNA in mouse spleen cells under constant temperature conditions in the different temperature group during postmortem intervals (PMIs) of 0–72 h. Thirty-nine mice were sacrificed by cervical dislocation and kept at constant temperatures of 10°C, 15°C, 20°C, 25°C, and 30°C. From 0 to 72 h after death, total RNA in spleen cells was extracted every 6 h. The cycle threshold (Ct) values of β-actin mRNA and 18S rRNA were obtained by real-time-quantitative polymerase chain reaction. The results showed that, under the conditions of different and constant temperatures after mouse death at 72 h, the Ct values of β-actin and 18S, Ct ratios of β-actin to 18S, and relative ratios of β-actin to 18S were significantly correlated with PMI. In addition, the relative degradation rates of β-actin and 18S appeared to change from fast to slow with the increase of temperature. By interpolation and fitting analysis of the data, we obtained a ternary quintic equation of the relationship between the change in the relative ratios and PMI, which can be used to infer PMI within a certain temperature range (10°C–30°C).


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