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ORIGINAL ARTICLE
Year : 2018  |  Volume : 4  |  Issue : 3  |  Page : 135-141

Differentially expressed microRNAs as potential markers for vital reaction of burned skin


1 Department of Forensic Pathology, School of Forensic Medicine, Southern Medical University, Guangzhou, China
2 Forensic Science Centre of Guangdong Provincial Public Security Department, Southern Medical University, Guangzhou, China
3 Department of Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China

Correspondence Address:
Dr. Qi Wang
Department of Forensic Pathology, School of Forensic Medicine, Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou, Guangdong
China
Dr. Xiao-Li Xie
Department of Toxicology, School of Public Health and Tropical Medicine, Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou, Guangdong
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jfsm.jfsm_1_18

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The identification of antemortem burns and postmortem burns is essential in forensic practice. In this study, microRNA (miRNA) microarray analysis was conducted to identify differentially expressed miRNAs in the skin of an experimental burn model. Microarray analysis revealed 24 differentially expressed miRNAs in antemortem burned mice skin, with 19 miRNAs significantly upregulated and 5 downregulated. Based on the intersection predicted using three databases (Targetscan, microRNA.org, and PITA), 293 potential miRNA targets were identified. These dysregulated miRNAs and their predicted targets were further analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Several functional categories and signaling pathways were enriched, including the “fc epsilon ri signaling pathway,” “endometrial cancer,” and “mTOR signaling pathway.” Expression patterns of 10 differentially expressed miRNAs were verified by reverse transcription-quantitative polymerase chain reaction in mice skins. The results agreed with the results of microarray analysis. These findings suggest that differentially expressed miRNAs revealed by microarray are potential markers for forensic molecular diagnosis of antemortem burns.


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