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ORIGINAL ARTICLE
Year : 2017  |  Volume : 3  |  Issue : 3  |  Page : 115-121

Cytochrome c oxidase subunit 1-based human RNA quantification to enhance mRNA profiling in forensic biology


1 Collaborative Innovation Center of Judicial Civilization, Key Laboratory of Evidence Science, Ministry of Education, China University of Political Science and Law, Beijing, China
2 National Center for Forensic Science, University of Central Florida, Orlando, Florida, United States of America

Correspondence Address:
Jack Ballantyne
National Center for Forensic Science, University of Central Florida, 12354 Research Parkway, Orlando, Florida 32826
United States of America
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jfsm.jfsm_63_17

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RNA analysis offers many potential applications in forensic science, and molecular identification of body fluids by analysis of cell-specific RNA markers represents a new technique for use in forensic cases. However, due to the nature of forensic materials that often admixed with nonhuman cellular components, human-specific RNA quantification is required for the forensic RNA assays. Quantification assay for human RNA has been developed in the present study with respect to body fluid samples in forensic biology. The quantitative assay is based on real-time reverse transcription-polymerase chain reaction of mitochondrial RNA cytochrome c oxidase subunit I and capable of RNA quantification with high reproducibility and a wide dynamic range. The human RNA quantification improves the quality of mRNA profiling in the identification of body fluids of saliva and semen because the quantification assay can exclude the influence of nonhuman components and reduce the adverse affection from degraded RNA fragments.


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